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Literature summary extracted from

  • Lewis, J.K.; Bruender, N.A.; Bandarian, V.
    QueE A radical SAM enzyme involved in the biosynthesis of 7-deazapurine containing natural products (2018), Methods Enzymol., 606, 95-118 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.99.3 overexpression in Escherichia coli Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin Bacillus subtilis
-
 7-carboxy-7-carbaguanine + NH3
-
 ?
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin Bacillus subtilis 168
-
 7-carboxy-7-carbaguanine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.99.3 Bacillus subtilis O31677
-
-
4.3.99.3 Bacillus subtilis 168 O31677
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.99.3 a single step purification under anoxic conditions, using the His6 affinity tag step suffices to purify sufficient protein for biochemical, structural, and spectroscopic studies Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin
-
 Bacillus subtilis 7-carboxy-7-carbaguanine + NH3
-
 ?
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin
-
 Bacillus subtilis 168 7-carboxy-7-carbaguanine + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.99.3 queE
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.99.3 4Fe-4S-center
-
 Bacillus subtilis
4.3.99.3 S-adenosyl-L-methionine S-adenosyl-L-methionine-dependent radical enzyme Bacillus subtilis